Coding

Part:BBa_K108026:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)

XylA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 294
    Illegal XhoI site found at 298
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 976


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

XylA was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).

References

1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.

2. NCBI.